Definition: The biosafety level presents different types of biocontainment safety measures used to separate dangerous biological microbes in laboratories. There are different biosafety levels ranging from biosafety level 1 (BSL-1) to biosafety level 4 (BSL-4) and these are categories from lowest to highest respectively. Introduction and Background Biosafety has implemented several sets of controlled principles, activities, advanced techniques, and organized practices used to prevent inadvertent and inadvertent exposure to hazardous agents, e.g., pathogens and toxicants, along with their accidental discharge/release. The term Biosafety indicates the protection provided to valuable biological materials by considering adequate control and accountability in order to eliminate and limit their unauthorized release, diversion along with their access and misuse. There are many unique and distinctive problems that can be encountered in animal rooms during animal activities. These animal rooms are better than microbiology laboratories because, due to the nature of the animals' activities, they may present unique and dangerous pathogens and toxicants that are not found in standard microbiology laboratories. Animals can generate dangerous agents through aerosols, including animal bites and scratches. Sometimes there is some zoonotic agent of other toxic agents that can be infected. Animal Biosafety Levels S. No Animal Biosafety Levels (ABSL) Practices Primary Barriers (Safety Equipment) Facilities (Secondary Barriers)1  Includes those organisms that are not hazardous and cause infectious disease. Present minimal potential risks to personnel and the environment.  Involves standard microbiological practices  No primary barrier  Mask, gown and gloves for eye and face protection required  Bench/instrument and sink2 required  Includes advanced practices, procedures and equipment along with auditing organized with ABSL facility- 1. This level includes moderate potentially hazardous agents causing moderate risk to personnel and low risk to the community.  Involves standard microbiological practices along with limited access Universal biohazard warning sign Instant precautions Medical surveillance policies  Primary barriers Physical containment Devices used for handling agents causing infectious materials, e.g. splashes Eye and face protection mask, gowns and gloves required Requires a biosafety cabinet ABSL-1 and availability of autoclaves3  Involves microbes that are infectious to both personnel and the environment. Causes a high risk to personnel and a moderate risk to the environment. Includes advanced practices, procedures and equipment along with control organized with the structure of ABSL-2. They can cause life-threatening illnesses through inhalation of the root of exposure.  Includes ABSL-2 practices and controlled access.  Decontamination of all contaminated waste