BENEDETTO'S SUGAR REDUCTION TEST Benedetto's Sugar Reduction Test causes color changes from blue to green precipitate and finally forms a brick red precipitate. In this case, Benedict's solution which is an aqueous solution of copper(II) sulfate, sodium carbonate and sodium citrate, used as a test for the presence of reducing sugar. Glucose is one of the reducing sugars. Aldehyde (CHO) and keto (C=O) functional groups are found in glucose. The Benedict test will detect the aldehyde and keto functional group. Since glucose has this functional group, Benedict's test shows positive results by changing the color of Benedict's reagent from blue to green, then to the brick-red precipitate. Therefore this test is semi-quantitative. This is because Benedict's reagent reduces copper(II) sulfate by forming a precipitate of copper(I) oxide upon heating. The blue color means there is no reducing sugar, the green precipitate represents less sugar, and the brick red precipitate means more sugars are formed. Thus, the intensity of the color indicates the amount of sugar.Cu2+ (blue) ...