Pain is a symptom of an illness or injury that occurs more frequently. Pain serves to remind, protect and facilitate the diagnosis of the disease. Pain is a protective mechanism for the body that is activated if body tissues are damaged1. Current analgesia-inducing drugs such as opioids and nonsteroidal anti-inflammatory drugs (NSAIDs) are not believed to be useful in all cases due to their side effects and low potency2. NSAIDs are associated with gastric irritation, bleeding, ulcers and perforation3. Consequently, the search for other alternatives becomes necessary. The history of medicinal plants is intimately connected with the history of civilization. According to the World Health Organization, in many countries around 80% of the population still uses medicinal plants for their primary healthcare due to poverty and lack of access to modern medicine4. Considering that the most important analgesic prototypes (e.g. salicylic acid and morphine) were originally derived from plant sources, studying plant species traditionally used as painkillers should be a fruitful research strategy in the search for new analgesic and anti-inflammatory drugs. to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essay Carica papaya is a medicinal plant native to Central America, which has spread to different parts of the world, Africa and Nigeria. It contains two main bioactive compounds, namely papain and chymopapain, which are used in the brewing, winemaking, textile and tanning industries6. Carica papaya is a medicinal plant known as it contains substances that can be used for therapeutic purposes. This plant has been recommended as antiulcerogenic, antiamoebic, antifungal, antimicrobial, antitumor, lipid-lowering and used in wound healing activity, free radical scavenging activity, diuretic activity, uterotonic activity and antifertility activity7 , 8. The Nsukka natives chew the dried seeds of Carica papaya to relieve bothersome headaches (migraines) and to reduce swollen wounds and reduce high blood pressure. Methanol seed extract of Carica papaya has been reported to have antinociceptive and anti-inflammatory activity in mice and rats9. Therefore, the present study was undertaken to evaluate the analgesic activity of aqueous extract of Carica Papaya Seeds in mice. Plant material and extraction: Four ripe and unripe fruits of Carica papaya were collected from a local market and authenticated. The papaya fruits were cut into pieces and the wet seeds were separated. These were then rinsed gently but thoroughly in tap water twice and air dried completely at room temperature for 4 weeks. The dried seeds were pulverized into a fine powder using a household grinder. 50 g of powdered seeds were boiled in 500 ml of distilled water for 30 minutes after which they were filtered using a clean piece of white cotton gauze. The filtrate was evaporated to complete dryness at 40°C, producing a fine, chocolate-colored solid residue with a sweet aroma (yield: 22.5%, w/w). The extraction process was repeated 4 times and the solid residue was weighed and collected in an airtight container stored in a refrigerator at 4°C. From this fresh preparation was prepared whenever required. Experimental animals: Mice weighing between 25 and 30 g were divided into 5 groups of 6 mice each. The animals were maintained on a standard diet a.