From the cut DNA lane, the digestion reaction worked, as evidenced by several visible bands in that lane, but was not 100% complete. The 1' stop lane is mostly one band, but also has several faint bands, meaning that the DNA fragment has started to bind together again. In the 10' stop lane, there is banding, which means that the fragments are largely tied back together. Summary and Discussion The restriction digest was not completed because the upper band is very large and is almost the same size as the uncut DNA. , which means that some DNA fragments have been digested and most of them are still intact. Also there are no bands at the end of the lane because the fragments are very large on their own. In the future, this may be improved by allowing more time for digestion during incubation and heating, or by using enzymes that have 100% activity instead of enzymes stored for a long time. The band pattern appeared to match the calculated one, but had some presumably missing bands, according to Table 1 below. On the gel there is a faint band at ~700 bp, a band at ~2000 bp, a band at ~4500 bp, and a band at ~7000 bp. These bands correspond to #7, #6, #4, and #3 in the left table, respectively. Table 1. Predicted band pattern when lambda DNA is cleaved by