IntroductionBacteria and archaea are all around us and in different environments, even if we are unable to see them without a microscope. One of the environments in which microorganisms live is the salty environment. The different types of microorganisms living in the saline environment are halotolerant, halophilic and non-halophilic. The halophilic microorganism lives and grows in environments with high salt content (Arai et al., 2014). There are also non-halophilic types that can tolerate salt but in small amounts, they would not survive in environments with a high concentration of salt, while halotolerant ones can survive in environments with high salt content, they can tolerate a little salt but grow better without the salt. saline environment (Brock et al., 2016). The microbes tested in this experiment apply to the previously mentioned saline, halotolerant, halophilic, and non-halophilic microorganisms. If several cultures with three different microorganisms were provided it is possible to determine the culture based on salinity and temperature. If 15% salt is used, the only expected results should be Halobacterium salinarium, if only 10% salt is used, the expected results should be Staphylococcus aureus, and if only 1% salt is used, the expected result should be serratia marcescense and staphylococcus aureus. Materials and methods First, the laboratory instructor provided a mixed broth containing H. salimarium, S, aureus, and S. marcescens. Additionally, three plates were provided, one of which contains 15% NaCl milk agar, the other contains 10% NaCl nutrient agar, and the last one contains 1% NaCl nutrient agar prepared before the laboratory. Using the broth mixture, a quadrant strip plate was made for each... paper half... optimally when the salt concentration is 15% and the temperature is 37°C (Grant, Kamekura, McGenity , & Ventosa, 2015). Serratia Marcescens grows best when the salt concentration is 1% and the temperature is 37°C. Staphylococcus Aureus grows optimally at a salt concentration of 1% and can tolerate up to 10% while the optimal temperature is 37°C. In the plates it was possible to obtain isolations of microorganisms. Two organisms were isolated in 1% NaCl and identified as Serratia Mercescens and S. Aureus. In 10% NaCl the only organism isolated was Staphylococcus Aureus. Halobactirium Salinarium 15% milk agar was isolated on the last plate. Using these plates which contain salt and milk agar it was possible to isolate two different types of microorganisms and in one of the plates it was possible to isolate two types of microorganisms.