Tissue culture has been practiced since the early 1900s. Since its inception, there have been many advances in producing a viable plant from culture. Tissue culture is defined as the growth of plants from plant tissue in an artificial medium and sterile environment. Uses of this technique include food processing, agriculture, pharmaceuticals and medicines. It has an influence on human well-being such as food processing, human health and environmental protection. There is a growing demand for tissue culture, so it is becoming more and more popular by exploring its commercial potential more. Tissue culture is also used for the production of pathogen-free plants, for germplasm preservation, and for year-round propagation. Callus culture: Callus formation is a mass of undifferentiated cells from an explant. Both auxins and cytokinins are necessary for proper development. Subculturing should be done every 3-5 weeks. This type of culture is mainly performed for the maintenance of cell lines or morphogenesis. Suspension culture: Cells are grown in a liquid medium to form a callus. Subculture should be performed every 3-14 days due to their faster growth rate than callus culture. This is the most used method in large-scale production. Single Cell Culture: Single cell culture produces a clone of identical cells under in vitro conditions. The leaf pieces are macerated in a mortar and pestle with a buffer, then centrifuged. The cells are then grown on nursing medium. Micropropagation: This method uses a mature cell to dedifferentiate into callus tissue. The two processes in micropropagation are known as organogenesis and somatic embryogenesis. Organogenesis develops shoot buds into apical meristems, while somatic embryogenesis forms non z...... center of paper ...... then disinfected in a 10% bleach solution for 15 minutes. It is important to place all materials needed for culture in the laminar flow hood to avoid the spread of contaminants once work begins. After thoroughly washing your hands and arms, remove the leaf from the bleach solution with sterile forceps, then resterilize the forceps and scalpel before cutting the petiole into 1cm pieces and the leaf into approximately 2cm squares. Place the explants on a Petri dish with media, pressing gently to ensure contact between explant and media. First the explants are placed on an initiating medium, then placed on a developing medium. The medium used depends on the growth stage and the requirement for salts, sugar, nutrients, vitamins, hormones and pH. This process ultimately produces many seedlings that can be transplanted into a non-sterile environment.